Recombinant Mouse Anti-RSV F Antibody (PABC-276)
CAT#: PABC-276
This product is a recombinant mouse antibody that can recognize RSV F. This antibody preferentially neutralizes subtype A viruses.
Tested Data
SDS-PAGE
Figure 1 Mouse Anti-RSV F Antibody (PABC-276) in SDS-PAGE
SDS-PAGE analysis of PABC-276 in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions.
HPLC
Figure 2 Recombinant Mouse Anti-RSV F Antibody (PABC-276) in HPLC
The purity of PABC-276 was greater than 95% as determined by SEC- HPLC.
ELISA
Figure 3 Recombinant Mouse Anti-RSV F Antibody (PABC-276) in ELISA
ELISA analysis of PABC-276 was performed by coating with Human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein / RSV-F Protein (His Tag).
The secondary antibody: Goat Anti-Mouse IgG-HRP
WB
Figure 4 Recombinant Mouse Anti-RSV F Antibody (PABC-276) in WB
Western blot analysis of PABC-276 was performed by Human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein / RSV-F Protein (His Tag).
PABC-276 incubation concentration: 2ng/μL.
The secondary antibody: Goat Anti-Mouse IgG-HRP
Lane1: Reducing antigen (0.1μg)
Lane2: Reducing antigen (0.3μg)
Lane2: Reducing antigen (0.6μg)
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Specifications
- Immunogen
- Recombinant adenovirus expressing RSV F
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- RSV F
- Species Reactivity
- HRSV (strain A2)
- Applications
- ELISA, Neut, DB, FC
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- The antibody 5C4 has been reported in applications of Enzyme-linked Immunosorbent Assay, Neutralization, Dot Blot, Flow Cytometry. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.
Target
- Alternative Names
- F; Fusion glycoprotein F0; RSV F; Respiratory Syncytial Virus
- UniProt ID
- P03420
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Precise in Neutralization Studies
The Recombinant Mouse Anti-RSV F Antibody has been indispensable in our lab for neutralization studies. Its specificity for RSV subtype A provides clear insights, helping us understand virus-host dynamics more comprehensively.
Reliable in ELISA Assays
Using this antibody in ELISA assays has given us consistent results with excellent sensitivity. The high-product purity ensures minimal background noise, making it a reliable choice for detecting RSV proteins in complex samples.
Efficient in Dot Blot Analysis
We've integrated this antibody in dot blot analyses with impressive results. It identifies RSV proteins with high specificity, contributing significantly to pathogen characterization projects in our lab.
Q&As
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What is the main target of the Recombinant Mouse Anti-RSV F Antibody?
A: The primary target of this antibody is the Fusion glycoprotein (F) of the Human Respiratory Syncytial Virus (HRSV), particularly strain A2. It recognizes the RSV F protein, which is crucial for the virus's fusion capability, making it a must-study protein in viral research.
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Can this antibody be used for detecting RSV in samples?
A: Yes, the Recombinant Mouse Anti-RSV F Antibody is suitable for various detection techniques including ELISA, flow cytometry, and Western blotting, making it versatile for identifying RSV presence in experimental samples.
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What are the recommended applications for this antibody?
A: This antibody is recommended for applications such as ELISA, dot blotting, flow cytometry, and neutralization assays. Researchers should optimize its usage based on specific assay requirements.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABC-276, RRID: AB_3111581)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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